Some Selected BioPack Sequences



ghnha parameters on 800

  • sw = 10,000
  • fold in D1 (2nd H1 dimension) with sw1 = 6,000
  • decouple N15 with dm2 = nny and ddf2 = ccg
  • use Watergate 3919 for H2O suppression
  • decouple CA and CO
  • d1 = 1 (shouldn’t it be 1.5?)
Processing with VNMR
  • remove solvent with filter (e.g., ssfilter=350)
  • left shift F1 frequency by -1200
  • LP forward in F2 and zero fill to 128
  • LP both forward and back in F1
  • setlp1(128,64,2)
  • check using 0,1,2,3 as 3rd parameter to see if back-LP is necessary
  • lp = lp1 = lp2 = 0

gnoesyChsqc paramater for 800

  • sw = sw1 = 10,000
  • fold in F2 (C13 dimension) with sw2 = 5300
  • recycle delay d1 = 0.75 (to keep H2O from recovering)
  • nt = 16 ; ni => 64 ; ni2 = 32
  • decouple C13 with dm = nnny and cccp using wurst80
  • dof at 35ppm (set to value returned by dofcal(35))
  • decouple N15 with dm2 = nyny and cccg
  • dof2 at 119ppm
  • Wet suppression in both Relax and Mix delays (shape = gauss)
Processing with VNMR
  • left shift F1 frequency by -1200 (?)
  • LP forward in F1
  • LP forward and back in F2
  • setlp2(64,32,3)
  • suggested final size 1024 x 256 x 128
  • solvent subtraction should be minimal (ss=~10)
  • lp = lp1 = lp2 = 0; rp = 0 and adjust if necessary