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Some Selected BioPack Sequences

ghnha parameters on 800

• sw = 10,000
• fold in D1 (2nd H1 dimension) with sw1 = 6,000
• decouple N15 with dm2 = nny and ddf2 = ccg
• use Watergate 3919 for H2O suppression
• decouple CA and CO
• d1 = 1 (shouldn’t it be 1.5?)

Processing with VNMR

• remove solvent with filter (e.g., ssfilter=350)
• left shift F1 frequency by -1200
• LP forward in F2 and zero fill to 128
• LP both forward and back in F1
• setlp1(128,64,2)
• check using 0,1,2,3 as 3rd parameter to see if back-LP is necessary
• lp = lp1 = lp2 = 0

gnoesyChsqc paramater for 800

• sw = sw1 = 10,000
• fold in F2 (C13 dimension) with sw2 = 5300
• recycle delay d1 = 0.75 (to keep H2O from recovering)
• nt = 16 ; ni => 64 ; ni2 = 32
• decouple C13 with dm = nnny and cccp using wurst80
• dof at 35ppm (set to value returned by dofcal(35))
• decouple N15 with dm2 = nyny and cccg
• dof2 at 119ppm
• Wet suppression in both Relax and Mix delays (shape = gauss)

Processing with VNMR

• left shift F1 frequency by -1200 (?)
• LP forward in F1
• LP forward and back in F2
• setlp2(64,32,3)
• suggested final size 1024 x 256 x 128
• solvent subtraction should be minimal (ss=~10)
• lp = lp1 = lp2 = 0; rp = 0 and adjust if necessary