Running NMR Experiments

It is not the purpose of this web site to train you to use the NMR. But we have assembled here some information that we deem to be useful to our students and users. Note: some of this information may become dated as software changes.

BioNMR (Proteins and Nucleic Acids)

BioPack provides the interface and pulse sequences for multidimensional NMR on proteins and nucleic acids, and most of the information on this web site pertains to the use of BioPack.

The general workflow for bioNMR is as follows:
  • insert sample and set temperature
  • tune the probe
  • lock on D2O
  • shim
  • find tof and referencing info
  • check pw90’s
  • load desired experiment, set parameters, and run
  • save your data and copy it to a thumb drive or FTP to another hard drive

Small Molecule NMR

ChemPack provides an ideal interface for NMR on small molecules. Below is a brief outline of using ChemPack
  • insert sample and tune probe (no need to retune if using similar samples)
  • Select solvent -> Find Z0
  • Gradient autoshim
  • Setup->Basic -> enter sample name (file name for saved dataset)

Additonal information information about running ChemPack 4 can be found here.

Getting Your Data

  • Make sure you save your data after each experiment (some setups may require you to give a name before even running the experiment).
  • Copy the data to your thumb drive, but make sure all the contents of the *.fid directory are copied over. The 500 sometimes has trouble with this.
  • Alternatively, use the Macintosh fetch program to FTP the data to the Mac workstation in the NMR room and then copy to your thumb drive.
  • For security reasons, it is not possible to retrieve your data from any other computer except for the Mac in the NMR room.

You are responsible

for retrieving your data. The data on the NMR computers are not backed up. Make sure you retrieve your all of your data.